Pk (V5) Epitope Tag (GKPIPNPLLGLDST) Mouse Monoclonal Antibody [Clone ID: SV5-PK1]

CAT#: SM1691X

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) mouse monoclonal antibody, clone SV5-PK1, Purified

Product Images

V5 tagged protein detected with Mouse anti V5-tag (Cat.-No SM1691)

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  V5 tagged protein detected with Mouse anti V5-Tag (Cat.-No SM1691))

Specifications

Product Data

• Clone Name: SV5-PK1
• Applications: ELISA, IHC, IP, R, WB
• Recommended Dilution: ELISA (1/1000-1/5000).
  Western blot (1/1000-1/5000).
  Immunoprecipitation.
  Immunofluorescence.
  Radioimmunoassays.
  Immunohistochemistry on Frozen Sections.
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Mice were infected with the paramyxovirus SV5, Simian-Virus 5. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 Ag14 myeloma cell line.
• Specificity: This antibody recognises a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5.
  This antibody has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined).
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography on Protein G
• Background: The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
• Note: This product is manufactued under an exclusive license from the University of St. Andrews,UK.