Tumor necrosis factor (TNF-alpha) Rat Monoclonal Antibody [Clone ID: MP6-XT22]

CAT#: SM1694LE

Tumor necrosis factor (TNF-alpha) rat monoclonal antibody, clone MP6-XT22, Low Endotoxin

Specifications

Product Data

• Clone Name: MP6-XT22
• Applications: ELISA, FC, FN, IHC
• Recommended Dilution: ELISA: 1-10 µg/ml.
  Functional Assays.
  Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 10⁶ cells in 100 µl (Membrane permeabilisation is required).
  Immunohistochemistry on Frozen Sections.
  This Clone is reported to work in Immunohistochemistry on Paraffin Sections.
• Reactivities: Mouse, Rat
• Host: Rat
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Murine TNF alpha expressed in E. coli.
• Specificity: This antibody is specific for tumour necrosis factor alpha (TNF alpha).
  Clone MP6-XT22 is reported to neutralise the effects of mouse TNF alpha (4,5).
• Formulation: PBS, pH 7.4 without preservatives.
  State: Low Endotoxin
  State: Liquid purified IgG fraction
  Stabilizer: None
  Preservative: None
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography on Protein G
• Background: TNF alpha is a 17 KDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF alpha forms dimers and trimers, and is also found as a 26KDa integral membrane protein. Mouse TNF alpha has cytolytic activity against many tumour cell lines, and has an important immuno-regulatory role.
• Synonyms: TNF, TNF-a, TNFA, TNFSF2, Cachectin