Tumor necrosis factor (TNF-alpha) Rat Monoclonal Antibody [Clone ID: MP6-XT22]

CAT#: SM1694P

Tumor necrosis factor (TNF-alpha) rat monoclonal antibody, clone MP6-XT22, Purified

Product Images

Recombinant Mouse TNF alpha detected with Rat anti Mouse TNF-alpha Antibody (Cat.-No SM1694P)

Specifications

Product Data

• Clone Name: MP6-XT22
• Applications: ELISA, FC, IHC
• Recommended Dilution: ELISA: 1-10 µg/ml.
  Flow Cytometry: Use 10 µl of 1/10-1/50 diluted antibody to label 10⁶ cells in 100 µl (Membrane permeabilisation is required).
  Immunohistochemistry on Frozen Sections.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Murine TNF-alpha expressed in E. coli.
• Specificity: This antibody is specific for tumour necrosis factor alpha (TNF alpha).
  Clone MP6-XT22 is reported to neutralise the effects of Mouse TNF alpha (4,5). We recommend the use of SM1694LE
• Formulation: PBS, pH 7.4
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography on Protein G
• Background: TNA alpha is a 17 KDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF alpha forms dimers and trimers, and is also found as a 26KDa integral membrane protein. Mouse TNF alpha has cytolytic activity against many tumour cell lines, and has an important immuno-regulatory role.
• Synonyms: TNF, TNF-a, TNFA, TNFSF2, Cachectin