Interferon gamma (IFNG) Mouse Monoclonal Antibody [Clone ID: D9D10]

CAT#: SM1701F

Interferon gamma (IFNG) mouse monoclonal antibody, clone D9D10, FITC

Specifications

Product Data

• Clone Name: D9D10
• Applications: FC
• Recommended Dilution: Flow cytometry: neat - 1/10; use 10µl of the suggested working dilution to label 10e6 cells in 100µl; Membrane permeabilisation is required for this application.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3X63-Ag-8.653 myeloma cell line.
• Specificity: This antibody recognises both natural and recombinant interferon gamma, binding only to the dimeric (functional) form.
• Formulation: PBS, pH7.2 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
  Label: FITC
  State: Liquid purified IgG
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: lot specific
• Purification: Ion exchange chromatography
• Conjugation: FITC
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: interferon, gamma
• Database Link:
  Entrez Gene 3458 Human
• Background: Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.
• Synonyms: IFN-gamma, gamma IFN