IL4 Rat Monoclonal Antibody [Clone ID: MP4-25D2]

CAT#: SM1702P

IL4 rat monoclonal antibody, clone MP4-25D2, Purified

Product Images

Western blot analysis of human IL-4 recombinant protein probed with RAT ANTI HUMAN INTERLEUKIN-4 followed by F (ab')2 RABBIT ANTI RAT IgG:HRP.

Specifications

Product Data

• Clone Name: MP4-25D2
• Applications: ELISA, FC, IHC, WB
• Recommended Dilution: Flow Cytometry: 1/10 - 1/50; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl; Membrane permeabilisation is required for this application.
  ELISA.
  Western Blot: 1/500 - 1/1000.
  Immunohistochemistry on frozen sections.
• Reactivities: Human
• Host: Rat
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified CHO cell expressed recombinant human IL-4.
• Specificity: This antibody is specific for Interleukin - 4 (IL-4). Clone MP4-25D2 is reported to neutralise IL-4 activity. We recommend the use of SM1702A for this purpose.
• Formulation: PBS, pH7.4 containing 0.09% Sodium Azide
  State: Purified
  State: Liquid purified IgG
• Concentration: lot specific
• Purification: Affinity chromatography on Protein G
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 3565 Human
• Background: IL-4 is a 20kD cytokine which is expressed by a subpopulation of activated T cells and mast cells.
• Synonyms: IL-4, BSF1, Lymphocyte stimulatory factor 1, Binetrakin, Pitrakinra