IL10 Rat Monoclonal Antibody [Clone ID: JES3-9D7]

CAT#: SM1703R

IL10 rat monoclonal antibody, clone JES3-9D7, PE

Specifications

Product Data

• Clone Name: JES3-9D7
• Applications: FC
• Recommended Dilution: Flow cytometry: Membrane permeabilisation is required for this application.
• Reactivities: Human
• Host: Rat
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: This antibody recognises IL-10 and may be used for the flow cytometric detection of intracellular IL-10.
• Formulation: PBS containing 0.09% Sodium Azide and 0.5% Bovine Serum Albumin
  Label: PE
  State: Lyophilized purified IgG
  Label: R. Phycoerythrin (RPE)
• Concentration: lot specific
• Conjugation: PE
• Storage: Store the antibody undiluted at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: 6 month from despatch.
• Gene Name: interleukin 10
• Database Link:
  Entrez Gene 3586 Human
• Background: Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. ILs have a variety of functions, but most function to direct other immune cells to divide and differentiate. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Human IL10 is a non glycosylated polypeptide consisting of 178 amino acids. There is 73% homology between the human and mouse IL10 proteins, however, the human IL10 acts on both human and mouse target cells, while the mouse IL10 has species specific activity. The cellular sources of IL10 are CD4+ T cells and T cell clones, thymocytes, B cells and B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL10 will stimulate the growth of stem cells, mast cells and thymocytes. IL10 enhances cytotoxic T cell development, and costimulates B cell differentiation and immunoglobulin secretion. IL10 inhibits cytokine production by macrophages and suppresses macrophage class II MHC expression. The human IL10 gene is on human chromosome 1.
• Synonyms: IL-10, CSIF, TGIF