Estrogen Receptor 1 (ESR1) Mouse Monoclonal Antibody [Clone ID: 6F11]

CAT#: SM1715

Estrogen Receptor 1 (ESR1) mouse monoclonal antibody, clone 6F11, Supernatant

Specifications

Product Data

• Clone Name: 6F11
• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1/50-1/100.
  Immunohistochemistry on Frozen Sections: 1/40-1/60. Zambonis fixative recommended for best results.
  Immunohistochemistry on Paraffin Sections: 1/40-1/80. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections; Sodium citrate buffer pH 6.0 is recommended for this purpose.
  Recommended Positive Control: Breast carcinoma.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human estrogen receptor (alpha form).
  Spleen cells from immunised mice were fused with cells of the mouse p3-NS1-Ag4-1 myeloma cell line.
• Specificity: This antibody recognises the Estrogen Receptor alpha chain.
  The Oestrogen Receptor status of breast cancer tissues is an important parameter in determining prognosis and response to therapy.
• Formulation: State: Supernatant
  State: Lyophilized Tissue Culture Supernatant containing 0.09% Sodium Azide
• Reconstitution Method: Restore with 1 ml distilled water
• Storage: Store the antibody undiluted at 2-8°C Prior to reconstitution
  and at -20°C Following reconstitution.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch
• Database Link:
  Entrez Gene 2099 Human
• Background: Estrogen Receptor alpha is a 65 kDa protein and a member of the steroid family of nuclear receptors. Estrogen Receptor alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, Estrogen Receptor alpha can positively or negatively regulate gene transcription.
  Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Estrogen Receptors play an important role in regulating mammary gland growth and differentiation. Breast tumors that are ER positive may also show a more favorable response to anti-estrogen therapies
• Synonyms: ER alpha, Estradiol receptor, ESR1, ESR, NR3A1