CD3E (Cytopl. Dom.) Rat Monoclonal Antibody [Clone ID: CD3-12]

CAT#: SM1754P

CD3E (Cytopl. Dom.) rat monoclonal antibody, clone CD3-12, Purified


USD 590.00

2 Weeks*

Size
    • 200 ug

Product Images

Specifications

Product Data
Clone Name CD3-12
Applications FC, IF, IHC, WB
Recommended Dilution Western Blot. 
Flow Cytometry: 1/50-1/100 (Membrane permeabilisation is required).
Immunohistochemistry on Frozen Sections: 1/1000. 
Immunohistochemistry on Paraffin Embedded Tissues: 1/1000.
Heat antigen retrieval treatment for 20 minutes prior to staining of paraffin sections is required (Tris/EDTA buffer pH 9.0 is recommended for this purpose).
Recommended Positive Control Tissue: Tonsil.
Reactivities Canine, Chicken, Equine, Human, Mouse, Porcine
Host Rat
Isotype IgG1
Clonality Monoclonal
Immunogen Synthetic peptide sequence derived from Cytoplasmic epitope of CD3
Specificity This antibody recognises a highly conserved epitope of the CD3 molecule expressed by T lymphocytes.
As Rat anti Human CD3, clone CD3-12 has been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species cross reactivity for the CD3 marker. (Jones et al. 1993; Kothlow et al. 2005).
Formulation PBS
State: Purified
State: Liquid purified IgG fraction from Tissue Culture Supernatant
Preservative: 0.09% Sodium Azide
Concentration lot specific
Purification Affinity Chromatography on Protein G
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Synonyms T3/Leu-4
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.