Integrin alpha E (ITGAE) Mouse Monoclonal Antibody [Clone ID: 2G5.1]

CAT#: SM1766P

Integrin alpha E (ITGAE) mouse monoclonal antibody, clone 2G5.1, Purified

Specifications

Product Data

• Clone Name: 2G5.1
• Applications: FC, IHC, IP
• Recommended Dilution: Flow cytometry: 1/50 - 1/100; use 10 µl of the suggested working dilution to label 5 x 10e5 cells in 100µl.
  Immunoprecipitation.
  Immunohistochemistry on frozen sections: 1/50 - 1/100.
  Recommended positive control tissue: intestine.
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human Intestinal lymphocyte. Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
• Specificity: This antibody recognises the CD103 cell surface antigen, also known as the alphaE integrin. This reacts with various subsets of intra-epithelial lymphocytes:- 40% of lamina propria T-cells; 30% of mesenteric lymphoblasts and some lymphocytes from other mucosa. It recognises less than 0.1% bone marrow cells and less than 0.7% PBL.
• Formulation: PBS containing 0.09% Sodium Azide and 0.1% Bovine Serum Albumin
  State: Purified
  State: Liquid purified IgG
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 3682 Human
• Background: CD103/Integrin alpha E is a new member of the integrin series of adhesion molecules containing the beta7 subunit. This antigen defines a developmentally important subset of T cells, namely mucosal T cells including all IEL (intraepithelial lymphocytes) and ~20% of lamina propria T cells. Expression of CD103 is more restricted outside these mucosal organs, appearing at lower levels on T cell subsets of the lymph node, dendritic epidermis and periphery. In non-epithelial CD103+ T cells there is a bias toward expression on CD8+ cells. Integrin alpha E is a heterodimer comprising a 150kDa alpha chain and a 120kDa beta chain. Integrin alpha E is expressed by approximately 1% of peripheral blood lymphocytes, activated T lymphocytes and by hairy cell leukaemia cells. The antigen is also expressed by intraepithelial lymphocytes.
• Synonyms: Integrin alpha-E, Integrin alpha-IEL, HML-1 antigen, ITGAE