CD59 Rat Monoclonal Antibody [Clone ID: YTH53.1]

CAT#: SM1768

CD59 rat monoclonal antibody, clone YTH53.1, Purified

Product Images

Staining of human peripheral blood granulocytes with RAT ANTI HUMAN CD59.

Specifications

Product Data

• Clone Name: YTH53.1
• Applications: FC, IHC, WB
• Recommended Dilution: Western Blot.
  Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 10e6 cells in 100 µl.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections: 1/5-1/10. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections; Sodium citrate buffer pH 6.0 is recommended for this purpose.
  Recommended Positive Control: Placenta.
  Removal of sodium azide is recommended prior to use in functional assays.
• Reactivities: Human
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human Peripheral Blood T cells. Spleen cells from an immunised DA rat were fused with cells of the Y3/Ag1.2.3 rat myeloma line.
• Specificity: This antibody recognises the CD59 cell surface antigen.
  Clone YTH53.1 has been shown to block the normal function of CD59.
• Formulation: PBS containing 0.09% Sodium Azide as preservative.
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 966 Human
• Background: CD59 is an 18-20kD GPI linked glycoprotein broadly expressed by human leucocytes and erythrocytes. CD59 is expressed by erythrocytes, and therefore staining in whole blood systems requires a higher concentration of antibody.
• Synonyms: MAC-inhibitory protein, Protectin, MEM43 antigen, MIC11, MIN1, MIN2, MIN3, MSK21, MACIF, MAC-IP, MIRL, HRF20, HRF-20