CD2 Mouse Monoclonal Antibody [Clone ID: LT2]

CAT#: SM1783LE

CD2 mouse monoclonal antibody, clone LT2, Azide Free

Product Images

Staining of Human peripheral blood lymphocytes with Mouse anti Human CD2 Antibody Azide Free, Low Endotoxin

Specifications

Product Data

• Clone Name: LT2
• Applications: FC, FN, IHC, IP
• Recommended Dilution: Flow Cytometry: Use 10 μl of 1/50-1/100 diluted antibody to label 10⁶ cells in 100 μl.
  Immunoprecipitation.
  Functional Studies.
  Immunohistochemistry on Frozen Sections: 1/200-1/400.
  The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of Acetone fixation for frozen sections.
  Recommended Positive Control: Human Tonsil.
• Reactivities: Chimpanzee, Human, Monkey
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Normal Human peripheral blood lymphocytes.
  Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line.
• Specificity: SM1783LE is a single chain transmembrane glycoprotein of 50kD, expressed by T cells, NK cells and thymocytes. CD2 is a receptor for CD58 (LFA-3), and is important in mediating the interaction between T cells and target cells or antigen presenting cells.
  This antibody inhibits NK cell mediated cytotoxicity, and ConA and PHA mediated blast transformation.
• Formulation: PBS, pH 7.4
  State: Azide Free
  State: Liquid purified IgG fraction (Low Endotoxin)
  Stabilizer: None
  Preservative: None
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD2 molecule
• Database Link:
  Entrez Gene 914 Human
• Background: CD2 which is also known as E-rosette receptor, T11 and lymphocyte-function antigen-2 (LFA-2) is expressed on T cells, thymocytes, and subset of natural killer cells. Human CD2 functions as the receptor for sheep erythrocytes, human CD58 (LFA-3), and CD15s (Sialyl Lewis X). p56lck, p59fyn, CD3eta and CD3epsilon are tyrosine-phosphorylated after CD2 stimulation. CD2 play a role in T cell activation, T- or NK-cell-mediated cytolysis, apoptosis in activated peripheral T cells, and regulation of T cell anergy.
• Synonyms: SRBC, Erythrocyte receptor, LFA-2, LFA-3 receptor, Rosette receptor
• Note: Does not work on paraffin sections.