CD8A Mouse Monoclonal Antibody [Clone ID: LT8]

CAT#: SM1784APC

CD8A mouse monoclonal antibody, clone LT8, APC

Product Images

Staining of human peripheral blood lymphocytes with Mouse Anti Human CD8-APC (SM1784APC).

Specifications

Product Data

• Clone Name: LT8
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 ul of neat antibody to label 10e6 cells or 100 ul human whole blood.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Normal human peripheral blood lymphocytes.
  Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line.
• Specificity: This antibody recognises the alpha-chain of the CD8 antigen, a 32kD glycoprotein expressed by the cytotoxicsuppressor subset of T cells and weakly by NK cells.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.
  Label: APC
  State: Lyophilized purified IgG fraction.
  Label: Allophycocyanin
• Reconstitution Method: Restore with 1 ml distilled water
• Purification: Ion Exchange Chromatography
• Conjugation: APC
• Storage: Prior to and following reconstitution store the antibody undiluted at 2-8°C.
  DO NOT FREEZE!
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens CD8a molecule (CD8A), transcript variant 1
• Database Link:
  Entrez Gene 925 Human
• Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
• Synonyms: CD8 alpha chain, CD8A, MAL

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
• Protein Pathways: Antigen processing and presentation, Cell adhesion molecules (CAMs), Hematopoietic cell lineage, Primary immunodeficiency, T cell receptor signaling pathway