CD45 (PTPRC) Mouse Monoclonal Antibody [Clone ID: F-10-89-4]

CAT#: SM1795APC

CD45 (PTPRC) mouse monoclonal antibody, clone F-10-89-4, APC

Product Images

Staining of human peripheral blood lymphocytes with Mouse anti Human CD45:APC (SM1795APC)

Specifications

Product Data

• Clone Name: F-10-89-4
• Applications: FC
• Recommended Dilution: Flow cytometry: Use 10 ul of neat antibody to label 10e6 cells or 100ul whole blood.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human T lymphocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
• Specificity: This antibody recognises the CD45 cell surface antigen, also known as the leucocyte common antigen (LCA). Clone F10-89-4 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes. This product is routinely tested in flow cytometry on human peripheral blood leucocytes
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
  Label: APC
  State: Lyophilized purified IgG
  Label: Allophycocyanin
• Purification: Affinity chromatography
• Conjugation: APC
• Storage: Prior to and following reconstitution store the antibody at 2-8°C.
  DO NOT FREEZE!
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 5788 Human
• Background: CD45 is a complex molecule existing in a number of isoforms.
  Antibodies recognising a common epitope on all of these isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200