CD45 (PTPRC) Mouse Monoclonal Antibody [Clone ID: F10-89-4]
Specifications
Product Data | |
Clone Name | F10-89-4 |
Applications | FC |
Recommended Dilution | Flow Cytometry: This product is routinely tested on human peripheral blood leucocytes. |
Reactivities | Human |
Host | Mouse |
Isotype | IgG2a |
Clonality | Monoclonal |
Immunogen | Human T lymphocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line. |
Specificity | This antibody recognises the CD45 cell surface antigen, also known as the leucocyte common antigen (LCA). Antibodies recognising a common epitope on all of these isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc. Clone F10-89-4 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes. |
Formulation | PBS, pH 7.4 Label: Biotin State: Liquid purified IgG fraction Stabilizer: 1% BSA Preservative: 0.09% Sodium Azide |
Concentration | lot specific |
Purification | Affinity Chromatography on Protein G |
Conjugation | Biotin |
Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Database Link | |
Background | CD45 is a complex molecule existing in a number of isoforms. |
Synonyms | PTPRC, Leukocyte common antigen, L-CA, T200 |
Reference Data |
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