CD45 (PTPRC) Mouse Monoclonal Antibody [Clone ID: F-10-89-4]

CAT#: SM1795R

CD45 (PTPRC) mouse monoclonal antibody, clone F-10-89-4, PE

Product Images

Staining of human peripheral blood lymphocytes with Mouse anti Human CD45 - RPE (SM1795R)

Specifications

Product Data

• Clone Name: F-10-89-4
• Applications: FC
• Recommended Dilution: Flow cytometry: use 10 µl of neat antibody to label 10e6 cells or 100 µl whole blood.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human T lymphocytes
• Specificity: SM1795R recognises the human CD45 cell surface antigen, also known as the leucocyte common antigen.
  Clone F10-89-4 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes.
• Formulation: PBS, containing 1% BSA , 5% Sucrose and 0.09% Sodium Azide
  Label: PE
  State: Lyophilized purified IgG fraction
  Label: R. Phycoerythrin (RPE)
• Reconstitution Method: Restore with 1.0 ml distilled water.
• Purification: Protein G affinity chromatography
• Conjugation: PE
• Storage: Prior to and following reconstitution store the antibody at 2-8°C.
  DO NOT FREEZE! Protect from light!
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 5788 Human
• Background: CD45 is a complex molecule existing in a number of isoforms.
  Antibodies recognising a common epitope on all of these isoforms are termed CD45 whilst those recognising
  only individual isoforms are termed CD45RA or CD45RO etc.
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200
• Note: This product is routinely tested in flow cytometry on human peripheral blood leucocytes.