CD45 (PTPRC) (CD45RA) Mouse Monoclonal Antibody [Clone ID: F8-11-13]
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Specifications
Product Data | |
Clone Name | F8-11-13 |
Applications | FC |
Recommended Dilution | Flow Cytometry (Neat -1/10): Use 10 µl to label 10e6 cells in 100 µl whole blood. |
Reactivities | Human |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Human T Lymphocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line. |
Specificity | This antibody recognises the human CD45RA cell surface antigen. Clone F8-11-13 reacts with the high molecular weight form of the leucocyte common antigen (LCA) expressed by B lymphocytes and a subset of T lymphocytes. |
Formulation | PBS, pH 7.2 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer. Label: FITC State: Liquid purified IgG fraction. Label: Fluorescein Isothiocyanate Isomer 1 |
Concentration | lot specific |
Purification | Affinity Chromatography on Protein G. |
Conjugation | FITC |
Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Database Link | |
Background | CD45 is a complex molecule existing in a number of isoforms. Antibodies recognising a common epitope on all of these isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc. |
Synonyms | PTPRC, Leukocyte common antigen, L-CA, T200 |
Reference Data |
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