CD45 (PTPRC) (CD45RB) Mouse Monoclonal Antibody [Clone ID: MEM-55]

CAT#: SM1797P

CD45 (PTPRC) (CD45RB) mouse monoclonal antibody, clone MEM-55, Purified


USD 475.00

5 Days*

Size
    • 200 ug

Product Images

Specifications

Product Data
Clone Name MEM-55
Applications FC, IHC, IP
Recommended Dilution Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 106 cells in 100 µl.
Immunoprecipitation.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Protein digestion pretreatment of paraffin sections not required. Antigen retrieval using heat treatment prior to staining of paraffin sections not required.
Reactivities Human, Primate
Host Mouse
Isotype IgG1
Clonality Monoclonal
Specificity This antibody recognizes the isoform of the leucocyte common antigen encoded by exon B and stains B cells, most T cells and monocytes.
Clone MEM-55 immunoprecipitates bands of 190, 200 and 205kDa. 
Formulation PBS
State: Purified
State: Liquid purified IgG fraction
Preservative: 0.09% Sodium Azide
Concentration lot specific
Purification Affinity Chromatography on Protein A
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
Synonyms PTPRC, Leukocyte common antigen, L-CA, T200
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.