CD45 (PTPRC) (CD45RB) Mouse Monoclonal Antibody [Clone ID: MEM-55]

CAT#: SM1797PT

CD45 (PTPRC) (CD45RB) mouse monoclonal antibody, clone MEM-55, Purified

Product Images

Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD45RB (SM1797P).

Specifications

Product Data

• Clone Name: MEM-55
• Applications: FC, IHC, IP, WB
• Recommended Dilution: Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 10⁶ cells in 100 µl.
  Immunoprecipitation.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
  Protein digestion pretreatment of paraffin sections not required. Antigen retrieval using heat treatment prior to staining of paraffin sections not required.
• Reactivities: Human, Primate, Rabbit
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: This antibody recognizes the isoform of the leucocyte common antigen encoded by exon B and stains B cells, most T cells and monocytes.
  Clone MEM-55 immunoprecipitates bands of 190, 200 and 205kDa.
  
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 5788 Human
• Background: CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200