CD45 (PTPRC) (CD45RB) Mouse Monoclonal Antibody [Clone ID: MEM-55]

CAT#: SM1797R

CD45 (PTPRC) (CD45RB) mouse monoclonal antibody, clone MEM-55, PE

Specifications

Product Data

• Clone Name: MEM-55
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 20 µl to label 10⁶ cells or 100 µl whole blood.
• Reactivities: Human, Primate
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human thymocytes and T lymphocytes
• Specificity: The antibody MEM-55 recognizes a siliadase-sensitive epitope of CD45RB, a 180-240 kDa single chain type I membrane glycoprotein, variant of CD45 (CD45RB isoform). CD45RB is expressed on a subset of T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells.
• Formulation: Phosphate buffered saline (PBS) containing 15 mM Sodium Azide and 0.2% (w/v) high-grade protease free BSA as a stabilizing agent.
  Label: PE
  State: Liquid purified Ig fraction
  Label: Conjugated with R-Phycoerythrin under optimum conditions. The conjugate is purified by Size-Exclusion Chromatography and adjusted for direct use
• Conjugation: PE
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 5788 Human
• Background: CD45RB is an of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RB is expressed e.g. in microglia and inflammatory cells.
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200