Integrin alpha E (ITGAE) Mouse Monoclonal Antibody [Clone ID: LF61]

CAT#: SM1800F

Integrin alpha E (ITGAE) mouse monoclonal antibody, clone LF61, FITC

Specifications

Product Data

• Clone Name: LF61
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 5 µl of neat-1/10 diluted antibody to label 10⁶ cells in 100 µl.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Hairy cell leukaemia cells.
  Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSO myeloma cell line.
• Specificity: This antibody recognizes the Human CD103 cell surface antigen. Other species not tested.
• Formulation: PBS
  Label: FITC
  State: Liquid purified IgG faction
  Stabilizer: 0.5% BSA
  Preservative: 0.09% Sodium Azide
  Label: Fluorescein Isothiocyanate Isomer 1
  Absorption emission: 490 nm / 525 nm
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: FITC
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  This product is photosensitive and should be protected from light.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 3682 Human
• Background: CD103/Integrin alpha E is a new member of the integrin series of adhesion molecules containing the beta7 subunit. This antigen defines a developmentally important subset of T cells, namely mucosal T cells including all IEL (intraepithelial lymphocytes) and ~20% of lamina propria T cells. Expression of CD103 is more restricted outside these mucosal organs, appearing at lower levels on T cell subsets of the lymph node, dendritic epidermis and periphery. In non-epithelial CD103+ T cells there is a bias toward expression on CD8+ cells. Integrin alpha E is a heterodimer comprising a 150kDa alpha chain and a 120kDa beta chain. Integrin alpha E is expressed by approximately 1% of peripheral blood lymphocytes, activated T lymphocytes and by hairy cell leukaemia cells. The antigen is also expressed by intraepithelial lymphocytes. It has recently been shown to be identical to the alpha E integrin.
• Synonyms: Integrin alpha-E, Integrin alpha-IEL, HML-1 antigen, ITGAE