CD180 / RP105 Rat Monoclonal Antibody [Clone ID: RP/14]

CAT#: SM1823PS

CD180 / RP105 rat monoclonal antibody, clone RP/14, Purified

Specifications

Product Data

• Clone Name: RP/14
• Applications: FC, FN
• Recommended Dilution: Flow cytometry: The typical starting working dilution is 1:50.
  Functional assays (B cell activation).
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Specificity: This antibody reacts with Radioprotective 105 (RP105, CD180).
• Formulation: PBS
  State: Purified
  State: Liquid 0.2 µm filtered Ig fraction
  Stabilizer: 0.1% bovine serum albumin
• Concentration: 0.1 mg/ml
• Purification: Protein G
• Background:

  
  Radioprotective 105 (RP105, CD180) is a type 1 transmembrane protein of 105 kDa with extracellular leucine-rich repeats (LLRs) and a short cytoplasmic tail. RP105, assigned as CD180, is similar to Drosophila Toll in the extracellular LLRs. Radioprotective 105 (RP105, CD180) expression is largely restricted to immune cells including CD19+ B cells and macrophages. Histological studies showed that RP105 is expressed mainly on mature B cells in mantle zones. Antibody-mediated (RP/14) cross-linking of RP105 induces resistance against irradiation-induced apoptosis, B-cell proliferation, and up-regulation of a costimulatory molecule B7.2, revealing RP105 as a potent regulator of B-cell activation. RP105 (CD180) has an important role in B-cell activation by LPS. It is important to note that RP105 associates with MD1, which is in this context important for RP105 with respect to B-cell surface expression and LPS recognition and signalling. MD-1 seems to be requisite for efficient expression of RP105.

• Synonyms: LY64