TRAIL (TNFSF10) (95-281) Mouse Monoclonal Antibody [Clone ID: 2E5]

CAT#: SM1847RP

TRAIL (TNFSF10) (95-281) mouse monoclonal antibody, clone 2E5, PE

Product Images

Apoptosis induced in JURKAT human T cell leukemia cell line by soluble recombinant human TRAIL is completely blocked by anti-human TRAIL (2E5). The neutralizing activity of the antibody 2E5 has been confirmed with various sources of soluble recombinant human TRAIL. A - medium B - recombinant TRAIL C - recombinant TRAIL + anti-human TRAIL (2E5; 0.06 ug/ml) D - recombinant TRAIL + anti-human TRAIL (2E5; 0.24 ug/ml) E - recombinant TRAIL + Isotype mouse IgG1 control

Specifications

Product Data

• Clone Name: 2E5
• Applications: Assay, FC
• Recommended Dilution: Flow cytometry.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant soluble fragment (aa 95-281) of human TRAIL
• Specificity: The antibody 2E5 reacts with TRAIL (APO-2L), a 21 kDa cytotoxic protein, activator of rapid apoptosis in tumor cells. TRAIL is mainly expressed in spleen, lung, prostate and also in many other tissues.
• Formulation: Phosphate buffered saline (PBS)
  Label: PE
  State: Liquid purified Ig fraction
  Stabilizer: 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA)
  Preservative: 15 mM sodium azide
  Label: Conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Concentration: 0.1 mg/ml
• Conjugation: PE
• Database Link:
  Entrez Gene 8743 Human
• Background: Human CD253 / TRAIL (TNF-Related Apoptosis Inducing Ligand), also called Apo2, is a type II membrane protein from the TNF family. TRAIL is a cytotoxic protein which activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL-induced apotosis, is achieved through binding to two dealth-signaling receptors, DR4 (CD261 / TRAIL-R1) and DR5 (CD262 / TRAIL-R2).
• Synonyms: Apo-2 ligand, TNFSF10, APO2L, Apo-2L