MHC Class I (monomorphic) Mouse Monoclonal Antibody [Clone ID: 2G5]

CAT#: SM1855F

MHC Class I (monomorphic) mouse monoclonal antibody, clone 2G5, FITC


USD 415.00

5 Days*

Size
    • 100 ug

Product Images

Specifications

Product Data
Clone Name 2G5
Applications FC
Recommended Dilution Flow Cytometry: Use 10 µl of Neat-1/5 diluted antibody to label 10e6 cells in 100 µl.
Reactivities Bovine, Guinea Pig, Hamster, Human, Mouse, Porcine, Rat
Host Mouse
Isotype IgG2b
Clonality Monoclonal
Immunogen Purified H-2Kb and H-2Db MHC-I molecules.
Spleen cells from immunised C1D mice were fused with cells of the X63 myeloma cell line.
Specificity This antibody recognises a monomorphic epitope on the MHC class I antigen. The epitope recognised by clone 2G5 is conformation dependent and is reported to be phylogenetically conserved.
Reactivity has been observed with some Canine samples suggesting that this antibody may recognise a polymorphic epitope of Canine MHC class 1.
Formulation PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.
Label: FITC
State: Liquid purified IgG fraction.
Label: Fluorescein Isothiocyanate Isomer 1
Concentration 0.1 mg/ml
Purification Affinity chromatography on Protein G
Conjugation FITC
Background MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.
Synonyms MHC Class 1, HLA Class 1
Reference Data

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.