MHC Class I (monomorphic) Mouse Monoclonal Antibody [Clone ID: 2G5]

CAT#: SM1855F

MHC Class I (monomorphic) mouse monoclonal antibody, clone 2G5, FITC

Specifications

Product Data

• Clone Name: 2G5
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of Neat-1/5 diluted antibody to label 10e6 cells in 100 µl.
• Reactivities: Bovine, Guinea Pig, Hamster, Human, Mouse, Porcine, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Purified H-2Kb and H-2Db MHC-I molecules.
  Spleen cells from immunised C1D mice were fused with cells of the X63 myeloma cell line.
• Specificity: This antibody recognises a monomorphic epitope on the MHC class I antigen. The epitope recognised by clone 2G5 is conformation dependent and is reported to be phylogenetically conserved.
  Reactivity has been observed with some Canine samples suggesting that this antibody may recognise a polymorphic epitope of Canine MHC class 1.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.
  Label: FITC
  State: Liquid purified IgG fraction.
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.1 mg/ml
• Purification: Affinity chromatography on Protein G
• Conjugation: FITC
• Background: MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.
• Synonyms: MHC Class 1, HLA Class 1