Anpep Rat Monoclonal Antibody [Clone ID: R3-63]

CAT#: SM1856F

Anpep rat monoclonal antibody, clone R3-63, FITC

Specifications

Product Data

• Clone Name: R3-63
• Applications: FC
• Recommended Dilution: Flow cytometry: neat - 1/5; use 10 µl of the suggested working dilution to label 10e6 cells in 100µl; The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Mouse intestinal APN. Spleen cells from immunised mice were fused with cells of the IR983F rat myeloma cell line.
• Specificity: This antibody recognises aminopeptidase N (APN), a cell surface protein homologous with human CD13. Clone R3-63 has been reported to block mouse APN enzyme activity. We recommend the use of SM1856A for this purpose.
• Formulation: PBS, pH7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
  Label: FITC
  State: Liquid purified IgG
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.1 mg/ml
• Purification: Affinity chromatography on Protein G
• Conjugation: FITC
• Gene Name: alanyl (membrane) aminopeptidase
• Database Link:
  Entrez Gene 16790 Mouse
• Background: In the mouse, CD13 is a non-covalently linked homodimer of approximately 150kD subunits expressed by a variety of cells including monocytes, macrophages, dendritic cell and veiled cells.
• Synonyms: Aminopeptidase N, ANPEP, APN, PEPN