Anpep Rat Monoclonal Antibody [Clone ID: R3-63]
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Specifications
Product Data | |
Clone Name | R3-63 |
Applications | FC |
Recommended Dilution | Flow Cytometry: 1/5; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl; The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. |
Reactivities | Mouse |
Host | Rat |
Isotype | IgG2a |
Clonality | Monoclonal |
Immunogen | Mouse intestinal APN. Spleen cells from immunised mice were fused with cells of the IR983F rat myeloma cell line. |
Specificity | This antibody recognises aminopeptidase N (APN), a cell surface protein homologous with human CD13. Clone R3-63 has been reported to block mouse APN enzyme activity. We recommend the use of SM1856A for this purpose. |
Formulation | PBS, pH7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin Label: FITC State: Liquid purified IgG Label: Fluorescein Isothiocyanate Isomer 1 |
Concentration | 0.5 mg/ml |
Purification | Affinity chromatography on Protein G |
Conjugation | FITC |
Gene Name | alanyl (membrane) aminopeptidase |
Database Link | |
Background | In the mouse, CD13 is a non-covalently linked homodimer of approximately 150kD subunits expressed by a variety of cells including monocytes, macrophages, dendritic cell and veiled cells. |
Synonyms | Aminopeptidase N, ANPEP, APN, PEPN |
Reference Data |
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