Anpep Rat Monoclonal Antibody [Clone ID: R3-63]

CAT#: SM1856RT

Anpep rat monoclonal antibody, clone R3-63, PE

Specifications

Product Data

• Clone Name: R3-63
• Applications: FC
• Recommended Dilution: Flow Cytometry Neat
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Mouse intestinal APN
• Specificity: MCA2183PE recognises mouse aminopeptidase N (APN), a cell surface protein homologous with human CD13. In the mouse, CD13 is a non-covalently linked homodimer of approximately 150kD subunits expressed by a variety of cells including monocytes, macrophages, dendritic cell and veiled cells.
  
  Clone R3-63 has been reported to block mouse APN enzyme activity (1). Serotec recommend the use of MCA2183XZ for this purpose.
• Formulation: Phosphate buffered saline pH7.4 0.09% Sodium Azide, 1% Bovine Serum Albumin,
  Label: PE
  State: Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
• Reconstitution Method: Reconstitute in 0.25 ml disilled water
• Concentration: IgG concentration 0.1 mg/ml
• Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Conjugation: PE
• Gene Name: alanyl (membrane) aminopeptidase
• Database Link:
  Entrez Gene 16790 Mouse
• Background: CD13 also known as Aminopeptidase N is a zinc dependent exopeptidase which cleaves amino acids from the N termini of target proteins including neuropeptides and cytokines. CD13 is expressed on all cells of the myeloid lineage as well as epithelial cells of the intestine, placenta, liver and kidney. CD13 is upregulated in a number of tumour types including lung, melanoma, thyroid and hepatocarcinoma and has been shown to correlate with poor prognosis. CD13 is a crucial molecule in tumour angiogenesis. CD13 is also the receptor for viruses from the coronavirus family.
• Synonyms: Aminopeptidase N, ANPEP, APN, PEPN