c-Myc (MYC) Mouse Monoclonal Antibody [Clone ID: 9E10]

CAT#: SM1863AP

c-Myc (MYC) mouse monoclonal antibody, clone 9E10, AP

Product Images

c-Myc tagged protein detected with Mouse anti c-Myc antibody AP conjugated.

Specifications

Product Data

• Clone Name: 9E10
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/500-1/5000.
  Western Blot: 1/100-1/500 (Non-Reducing Conditions).
  Detects a band of approximately 62kDa in Colo 320HSR cell lysates.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections: This product does not require protein digestion pre-treatment of paraffin sections prior to staining. It does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region (residues 408-439) of Human c-myc conjugated to KLH.
  Spleen cells from immunised Balb/c mice were fused with cells of the myeloma cell line.
• Specificity: This clone 9E10 antibody detects the 62kDa c-myc gene product, which is involved in the regulation of the cell cycle and cell growth.
  This antibody may also be used to detect the commonly used c-Myc 'Tag'.
  This clone 9E10 antibody recognizes the sequence EQKLISEEDL and may be used to detect proteins and peptides labelled with molecular tags containing this sequence (Hilpert et al. 2001).
• Formulation: PBS
  Label: AP
  State: Liquid purified IgG fraction from tissue culture supernatant
  Preservative: 0.09% Sodium Azide
  Label: Alkaline Phosphatase
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: AP
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  Should this product contain a precipitate we recommend microcentrifugation before use.
• Stability: Shelf life: One year from despatch.
• Gene Name: Homo sapiens v-myc avian myelocytomatosis viral oncogene homolog (MYC)
• Database Link:
  Entrez Gene 4609 Human
• Background: p62^c-myc is primarily located to the cell nucleus, but has also been shown to localised to the cytoplasm in several cell lines. Overexpression of c-myc has been reported in a wide variety of human cancers.
• Synonyms: myc tag, myc-tag, c-myc tag

Reference Data

• Protein Families: Druggable Genome, Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - TGFb/BMP signaling pathway, Stem cell relevant signaling - Wnt Signaling pathway, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Jak-STAT signaling pathway, MAPK signaling pathway, Pathways in cancer, Small cell lung cancer, TGF-beta signaling pathway, Thyroid cancer, Wnt signaling pathway