c-Myc (MYC) Mouse Monoclonal Antibody [Clone ID: 9E10]
Product Images
![](https://cdn.origene.com/img/defaults-img.jpg)
Other products for "MYC"
Specifications
Product Data | |
Clone Name | 9E10 |
Applications | ELISA, FC, IHC, IP, WB |
Recommended Dilution | ELISA: 1/100-1/500. Western Blot: 1/100-1/500 (Non-Reducing Conditions). Detects a band of approximately 62kDa in Colo 320HSR cell lysates. Flow Cytometry: Use 10 µl of Neat-1/10 diluted antibody to label 1x10e6 cells in 100 µl. Membrane permeabilisation is required. Immunohistochemistry on Frozen and Paraffin Sections: This product does not require protein digestion pre-treatment of paraffin sections prior to staining. It does not require antigen retrieval using heat treatment prior to staining of paraffin sections. |
Reactivities | Human |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Synthetic peptide sequence AEEQKLISEEDLL corresponding to the C-terminal region (residues 408-439) of Human c-myc conjugated to KLH. Spleen cells from immunised Balb/c mice were fused with cells of the myeloma cell line. |
Specificity | This antibody detects the 62kDac-myc gene product, which is involved in the regulation of the cell cycle and cell growth. This antibody may also be used to detect the commonly used c-myc tag. |
Formulation | PBS containing 0.09% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction. |
Concentration | lot specific |
Purification | Affinity Chromatography on Protein G. |
Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Gene Name | Homo sapiens v-myc avian myelocytomatosis viral oncogene homolog (MYC) |
Database Link | |
Background | p62c-myc is primarily located to the cell nucleus, but has also been shown to localised to the cytoplasm in several cell lines. Overexpression of c-myc has been reported in a wide variety of human cancers. |
Synonyms | myc tag, myc-tag, c-myc tag |
Reference Data | |
Protein Families | Druggable Genome, Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - TGFb/BMP signaling pathway, Stem cell relevant signaling - Wnt Signaling pathway, Transcription Factors |
Protein Pathways | Acute myeloid leukemia, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Jak-STAT signaling pathway, MAPK signaling pathway, Pathways in cancer, Small cell lung cancer, TGF-beta signaling pathway, Thyroid cancer, Wnt signaling pathway |
Documents
Product Manuals |
FAQs |
{0} Product Review(s)
0 Product Review(s)
Submit review
Be the first one to submit a review
Product Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.