CD177 Mouse Monoclonal Antibody [Clone ID: MEM-166]

CAT#: SM2003

CD177 mouse monoclonal antibody, clone MEM-166, Purified

Product Images

Staining of Human peripheral blood granulocytes with Mouse Anti Human CD177 Antibody (Cat.-No SM2003).

Specifications

Product Data

• Clone Name: MEM-166
• Applications: FC, IP, WB
• Recommended Dilution: Western blot (Non-reducing conditions).
  Immunoprecipitation.
  Flow Cytometry: Use 10µl of 1/50-1/100 diluted CD177 antibody to label 10⁶ cells in 100µl.
• Reactivities: Human, Rhesus Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: This antibody recognizes CD177 (Neutrophil Glycoprotein NB1).
  Antibodies against NB1 can be involved in neonatal alloimmune neutropenia (NAN).
  MEM-166 recognizes CD177 under Non-Reducing Conditions.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography on Protein G
• Database Link:
  Entrez Gene 57126 Human
• Background: CD177 (NP1), a glycosyl phosphatidylinositol (GPI) linked N glycosylated cell surface glycoprotein, was first described in a case of neonatal alloimmune neutropenia and analyses showed cell surface expression of a 60 kDa protein, 14 kDa greater than the predicted size, probably due to the presence of 3 potential N glycosylation sites. Immunohistochemistry demonstrated expression in bone marrow early erythroblasts, megakaryocytes, promyelocytes, and myelocytes. The neutrophil NB1 antigen (CD177) is expressed by 97% of the caucasian population.
• Synonyms: NB1, PRV1, HNA-2a, HNA2a