MRP8 / MRP14 Mouse Monoclonal Antibody [Clone ID: MAC387]

CAT#: SM2011F

MRP8 / MRP14 mouse monoclonal antibody, clone MAC387, FITC


USD 415.00

5 Days*

Size
    • 100 ug

Product Images

Specifications

Product Data
Clone Name MAC387
Applications FC
Recommended Dilution Flow Cytometry: Use 10 µl of neat antibody to label 10e6 cells in 100 µl.
(Membrane permeabilization is required).
Reactivities Bovine, Canine, Equine, Feline, Guinea Pig, Human, Monkey, Porcine, Rabbit, Rat
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Human Monocytes.
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Specificity This antibody recognises the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain.
The antigen recognized by this anti Macrophages antibody is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia.
Formulation PBS containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.
Label: FITC
State: Liquid purified IgG fraction
Label: Fluorescein Isothiocyanate Isomer 1
Concentration 0.1 mg/ml
Purification Affinity Chromatography on Protein G
Conjugation FITC
Background Macrophages comprise of many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and promonocyte states of the monocyte stage, they enter the blood, where they circulate for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content becomming macrophages. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes including lysozyme and collagenases, several complement components and coagulation factors, some prostaglandins and leukotrienes, and many regulatory molecules (Interferon, Interleukin 1). Among cells that are now recognised as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues).
Synonyms S100A8/A9, S100A8, S100A9, MRP8/14
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.