Clec7a Rat Monoclonal Antibody [Clone ID: 2A11]

CAT#: SM2025B

Clec7a rat monoclonal antibody, clone 2A11, Biotin

Product Images

Staining of mouse peripheral blood granulocytes with RAT ANTI MSE BETA GLUCAN RECEPTOR:BIOTIN.

Specifications

Product Data

• Clone Name: 2A11
• Applications: FC
• Recommended Dilution: Flow Cytometry: Neat - 1/10; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Dectin-1 transfected NIH3T3 cells and recombinant soluble Dectin-1. Spleen cells from immunised Fischer rats were fused with cells of the rat Y3 myeloma cell line.
• Specificity: This antibody recognises beta-glucan receptor, also known as Dectin-1. This antibody inhibits the binding of zymosan to macrophages via the beta-glucan receptor. The low endotoxin version (SM2025LE) is recommended for functional studies.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
  Label: Biotin
  State: Liquid purified IgG
• Concentration: 0.1 mg/ml
• Purification: Affinity chromatography on Protein G
• Conjugation: Biotin
• Database Link:
  Entrez Gene 56644 Mouse
• Background: Dectin-1 is predominantly expressed by cells of the monocyte/macrophage and neutrophil lineages, but also at lower levels by dendritic cells and a subpopulation of T cells. As a major leucocyte receptor for beta-glucan this molecule may have a key role in the immunomodulatory effects of beta-glucans and in the host response to fungal pathogens. Recent work has shown that Dectin-1 may stimulate reactive oxygen production in macropahges via the protein tyrosine kinase known as Syk.
• Synonyms: Beta-glucan receptor, BGR, CLECSF12, DECTIN1