Clec7a Rat Monoclonal Antibody [Clone ID: 2A11]

CAT#: SM2025R

Clec7a rat monoclonal antibody, clone 2A11, PE

Specifications

Product Data

• Clone Name: 2A11
• Applications: FC
• Recommended Dilution: Flow Cytometry (Neat -1/10): Use 10 ul of the suggested working dilution to label 10e6 cells in 100 ul.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Dectin-1 transfected NIH3T3 cells and recombinant soluble Dectin-1.
  Spleen cells from immunised Fischer rats were fused with cells of the rat Y3 myeloma cell line2
• Specificity: Recognises Murine Beta-Glucan Receptor (Dectin-1).
  This antibody inhibits the binding of zymosan to macrophages via the beta-glucan receptor. The low endotoxin version SM2025LE is recommended for Functional Studies.
• Formulation: PBS containing 0.09% Sodium Azide as preservative and 1% BSA and 5% sucrose as stabilizers.
  Label: PE
  State: Lyophilized purified IgG fraction.
  Label: R. Phycoerythrin (RPE)
• Reconstitution Method: Restore with 1 ml distilled water
• Purification: Affinity Chromatography on Protein G.
• Conjugation: PE
• Database Link:
  Entrez Gene 56644 Mouse
• Background: Dectin-1 is predominantly expressed by cells of the monocyte/macrophage and neutrophil lineages, but also at lower levels by dendritic cells and a subpopulation of T cells.
  As a major leucocyte receptor for beta-glucan this molecule may have a key role in the immunomodulatory effects of beta-glucans and in the host response to fungal pathogens.
  Recent work has shown that Dectin-1 may stimulate reactive oxygen production in macropahges via the protein tyrosine kinase known as Syk.
• Synonyms: Beta-glucan receptor, BGR, CLECSF12, DECTIN1