CD45 (CD45R) Mouse Monoclonal Antibody [Clone ID: 20.96]

CAT#: SM2030R

CD45 (CD45R) mouse monoclonal antibody, clone 20.96, PE

Specifications

Product Data

• Clone Name: 20.96
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of neat antibody to label 1 x 10⁶ cells in 100 µl.
• Reactivities: Sheep
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Ovine thymocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
• Specificity: This antibody recognises the CD45R cell surface antigen.
• Formulation: PBS, pH 7.4
  Label: PE
  State: Lyophilized purified IgG fraction from tissue culture supernatant
  Stabilizer: 1% BSA, 5% Sucrose
  Preservative: 0.09% Sodium Azide
  Label: R. Phycoerythrin (RPE)
• Purification: Affinity Chromatography on Protein G
• Conjugation: PE
• Background: CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
• Synonyms: Leukocyte common antigen, L-CA, PTPRC, T200, CD45R