MHC Class II DQ/DR (polymorphic) Mouse Monoclonal Antibody [Clone ID: 28.1]

CAT#: SM2032R

MHC Class II DQ/DR (polymorphic) mouse monoclonal antibody, clone 28.1, PE

Product Images

Staining of sheep peripheral blood lymphocytes with MOUSE ANTI OVINE MHC CLASS II DQ/DR:RPE (SM2032R).

Specifications

Product Data

• Clone Name: 28.1
• Applications: FC
• Recommended Dilution: Flow Cytometry: Neat - 1/10; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
• Reactivities: Sheep
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Ovine alveolar macrophages. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
• Specificity: This antibody recognises a polymorphic epitope on MHC class II DQ and DR molecules. In recent work, this clone was found to recognise ovine MHC II transfectants DQ - T28.1, DQ - T26.2 and DR - T31.3 but not DR - T8.1. (Ballingall, K. et al. 1995).
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
  Label: PE
  State: Lyophilized purified IgG
  Label: R. Phycoerythrin (RPE)
• Reconstitution Method: Restore with 1 ml distilled water
• Purification: Affinity chromatography on Protein G
• Conjugation: PE
• Background: Ovine MHC class II antigens are expressed on several cell types, including B cells, activated T cells, monocytes, macrophages and dendritic cells.