Junctional Adhesion Molecule 1 (F11R) Mouse Monoclonal Antibody [Clone ID: BV16]

CAT#: SM2041F

Junctional Adhesion Molecule 1 (F11R) mouse monoclonal antibody, clone BV16, FITC

Specifications

Product Data

• Clone Name: BV16
• Applications: FC, IF, IHC, WB
• Recommended Dilution: Immunohistochemistry on frozen sections: The typical starting working dilution is 1:50.
  Flow cytometry: The typical starting working dilution is 1:50.
  Immunoflourescence.
  Western blot: The typical starting working dilution is 1:50.
  Does not work in Immunoprecipitation.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Fusion protein consisting of the extracellular domain of human JAM and the Fc portion of human IgGs
• Specificity: The monoclonal antibody BV16 recognizes the human junction adhesion molecule (JAM)-A.
• Formulation: PBS
  Label: FITC
  State: Liquid 0.2 µm filtered Ig fraction
  Stabilizer: 1% bovine serum albumin
  Preservative: 0.02% sodium azide
• Concentration: 0.1 mg/ml
• Purification: Potein G
• Conjugation: FITC
• Database Link:
  Entrez Gene 50848 Human
• Background: Together with JAM-C (JAM-2) and JAM-B (VE-JAM or JAM-3), JAM-A belongs to a family of adhesion proteins with a V-C2 immunoglobulin domain organization and their molecular weight is about 30-40 kDa. JAMs are important for a variety of cellular processes, including tight junction assembly, leukocyte transmigration, platelet activation, angiogenesis and virus binding. JAM-A is expressed by endothelial and epithelial cells, platelets, neutrophils, monocytes, lymphocytes and erythrocytes. Like all other JAMs, JAM-A plays an important role in tight junctions where it is involved in cell-to-cell adhesion through homophilic interaction. It codistributes with other tight junction components as ZO-1, 7H6 antigen, cingulin and occludin. JAM-A also plays an important role in leukocyte transmigration. Leukocyte transmigration can be blocked by antibodies and by soluble JAM-A/Fc fusion proteins. Homophilic JAM-A interactions between leukocytes and the endothelium but also heterophilic interactions of JAM-A with the beta2-integrin leukocyte function-associated antigen-1 (LFA-1) are considered to actively guide leukocytes during transmigration. Several studies imply a role for JAM-A in the initiation of atherosclerosis since JAM-A is upregulated on early atherosclerotic endothelium. The adhesion of activated platelets on the activated endothelium is mediated by homophilic interactions of JAM-A.
• Synonyms: JAM-A, Platelet F11 receptor, F11R, JCAM, PAM1