CD45 Mouse Monoclonal Antibody [Clone ID: 1.11.32]

CAT#: SM2055F

CD45 mouse monoclonal antibody, clone 1.11.32, FITC

Specifications

Product Data

• Clone Name: 1.11.32
• Applications: FC
• Recommended Dilution: Flow Cytometry: Neat - 1/5; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
• Reactivities: Bovine, Goat, Sheep
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Ovine efferent lymphatic duct lymphocytes
• Specificity: This antibody recognises the CD45 (Leucocyte common antigen), which is expressed on all ovine lymphocytes, macrophages and granulocytes.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
  Label: FITC
  State: Liquid purified IgG
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.1 mg/ml
• Purification: Affinity chromatography on Protein G
• Conjugation: FITC
• Background: CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
• Synonyms: Leukocyte common antigen, L-CA, PTPRC, T200, CD45RB, CD45RO, CD45RA, CD45R