CD4 Mouse Monoclonal Antibody [Clone ID: 2-35]

CAT#: SM2066F

CD4 mouse monoclonal antibody, clone 2-35, FITC

Specifications

Product Data

• Clone Name: 2-35
• Applications: FC
• Recommended Dilution: Flow Cytometry: Neat - 1/10; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
• Reactivities: Chicken
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Chicken embryonic thymocytes. Lymph node cells from immunised Balb/c mice were fused with cells of the SP2/0 myeloma cell line.
• Specificity: This antibody recognises the homologue of human CD4, a 64kD cell surface protein expressed by thymocytes and a subset of T cells.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
  Label: FITC
  State: Liquid purified IgG
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.1 mg/ml
• Purification: Affinity chromatography on Protein G
• Conjugation: FITC
• Background: CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.
• Synonyms: T-cell surface antigen T4/Leu-3