IL2 Rat Monoclonal Antibody [Clone ID: MQ1-17H12]

CAT#: SM2088F

IL2 rat monoclonal antibody, clone MQ1-17H12, FITC

Specifications

Product Data

• Clone Name: MQ1-17H12
• Applications: FC
• Recommended Dilution: Flow cytometry (neat - 1/10): Use 10 µl of the suggested working dilution to label 10e6 cells in 100 µl. Membrane permeabilisation is required for this application.
• Reactivities: Human, Monkey
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Specificity: This antibody recognises interleukin-2 (IL-2) and may be used to detect intracellular IL-2 in flow cytometric techniques.
• Formulation: PBS, pH 7.4, containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
  Label: FITC
  State: Liquid purified IgG
• Concentration: lot specific
• Purification: Affinity chromatography on Protein G
• Conjugation: FITC
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Protect from light.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: interleukin 2
• Database Link:
  Entrez Gene 3558 Human
• Background: Interleukin 2 (IL2) is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. IL2 has been shown to have antitumor effects in some studies. This is probably mediated by cytotoxic effector cells.
• Synonyms: IL-2, TCGF