NKG2A/C/E Rat Monoclonal Antibody [Clone ID: 20d5]

CAT#: SM2091R

NKG2A/C/E rat monoclonal antibody, clone 20d5, PE

Specifications

Product Data

• Clone Name: 20d5
• Applications: FC
• Recommended Dilution: Flow Cytometry: Neat - 1/2; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl; The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: CHO transfected cells expressing the B6 allele of NKG2A.
  Spleen cells from immunised Lewis rats were fused with cells of the mouse P3X63-Ag8.653 myeloma cell line.
• Specificity: This antibody recognises NKG2A, NKG2C and NKG2E, which are isoforms of the NKG2 lectin-like family.
  Clone 20d5 is reported to block ligand binding to the receptor. We recommend the use of SM2091LE for this purpose.
• Formulation: PBS, pH7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin.
  Label: PE
  State: Lyophilized purified IgG fraction.
  Label: R. Phycoerythrin (RPE)
• Reconstitution Method: Restore with 1 ml distilled water
• Concentration: 0.1 mg/ml
• Purification: Affinity Chromatography on Protein G.
• Conjugation: PE
• Background: In mice, NKG2 subunits associate with CD94 to form heterodimers at the surface of natural killer (NK) cells. The CD94/NKG2 heterodimer is the receptor for a non-classical MHC class I ligand, which is Qa-1 in the mouse.
• Synonyms: CD159a, CD159c, CD159e, NKG2 lectin-like family