Itga4 Rat Monoclonal Antibody [Clone ID: 9C10 (MFR.4.B)]

CAT#: SM2092F

Itga4 rat monoclonal antibody, clone 9C10 (MFR.4.B), FITC

Product Images

Staining of mouse peripheral blood lymphocytes with RAT ANTI MOUSE CD49d: FITC.

Specifications

Product Data

• Clone Name: 9C10 (MFR.4.B)
• Applications: FC
• Recommended Dilution: Flow Cytometry: Neat - 1/2; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl; The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
• Reactivities: Human, Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Mouse LyD9 haematopoietic progenitor cells. Spleen cells from immunised Lewis rats were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.
• Specificity: This antibody recognises alpha 4 integrin (CD49d). Clone 9C10 (MFR4.B) is reported to significantly inhibit binding of CD49d to CD106 but only when used in combination with clone R1-2.
• Formulation: PBS, pH7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
  Label: FITC
  State: Liquid purified IgG
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.1 mg/ml
• Purification: Affinity chromatography on Protein G
• Conjugation: FITC
• Database Link:
  Entrez Gene 16401 Mouse
• Background: CD49d is a 150kD glycoprotein that can associate with either beta 1 integrin (CD29) or beta 7 integrin to form heterodimers CD49d/CD29 (VLA-4) and alpha4/beta7 (LPAM-1) respectively. CD49d is expressed on most lymphocytes, granulocytes, monocytes and thymocytes. The primary ligands for CD49d are CD106 (VCAM-1), fibronectin and MAdCAM-1.
• Synonyms: Integrin alpha-4, Integrin alpha-IV, VLA-4, VLA4