Rat Lambda light chain Mouse Monoclonal Antibody [Clone ID: MARL-15]

CAT#: SM2096HRP

Rat Lambda light chain mouse monoclonal antibody, clone MARL-15, HRP

Specifications

Product Data

• Clone Name: MARL-15
• Applications: ELISA
• Recommended Dilution: ELISA.
• Reactivities: Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Rat lambda light chain. Spleen cells from immunised BALB/c mice were fused with cells of the rat PAI-O myeloma cell line.
• Specificity: This antibody recognises immunoglobulin lambda light chain. This antibody shows no cross-reactivity to Ig heavy chains or to kappa light chain.
• Formulation: PBS, pH7.4 containing 0.01% Thiomersal and 50% Glycerol
  Label: HRP
  State: Liquid purified IgG
  Label: Horseradish Peroxidase
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography
• Conjugation: HRP
• Background: All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).