CD49e / ITGA5 Rat Monoclonal Antibody [Clone ID: MFR5 (5H10)]

CAT#: SM2100F

CD49e / ITGA5 rat monoclonal antibody, clone MFR5 (5H10), FITC

Product Images

Staining of Mouse peripheral blood lymphocytes with Rat Anti Mouse CD49e antibody FITC conjugated (SM2100F).

Specifications

Product Data

• Clone Name: MFR5 (5H10)
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of neat antibody to label 10e6 cells in 100 µl.
  The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Mouse MC/9 cell line.
• Specificity: This antibody recognises Integrin alpha 5 (CD49e), which associates with the beta 1 integrin (CD29) to form the fibronectin receptor CD49e/CD29 (VLA-5).
  Clone MFR5(5H10) has been reported to block CD49e mediated function. We recommend the use of SM2100LE for this purpose.
• Formulation: PBS containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer
  Label: FITC
  State: Liquid purified IgG fraction
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.1 mg/ml
• Purification: Affinity Chromatography on Protein G
• Conjugation: FITC
• Background: Integrin alpha 5 belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post translational cleavage in the extracellular domain to yield disulfide linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell surface mediated signalling. It is expressed on thymocytes, activated T cells, mast cells, and a variety of mouse cell lines.
• Synonyms: Integrin alpha-5, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, VLA5, FNRA