CD52 Rat Monoclonal Antibody [Clone ID: YTH34.5]

CAT#: SM2135F

CD52 rat monoclonal antibody, clone YTH34.5, FITC

Product Images

Staining of human peripheral blood lymphocytes with RAT ANTI HUMAN CD52:FITC

Specifications

Product Data

• Clone Name: YTH34.5
• Applications: FC
• Recommended Dilution: Flow Cytometry (Neat): Use 10 ul of the suggested working dilution to label 10e6 cells or 100 ul whole blood.
• Reactivities: Human, Monkey
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human lymphocytes.
• Specificity: This antibody reacts with the human CD52 antigen, also known as CAMPATH-1.
  Humanised versions of CAMPATH-1 specific antibodies are currently in clinical trials for the treatment of a range of lymphoid malignancies.
• Formulation: PBS containing 0.09% Sodium Azide as preservative and 1% Bovine Serum Albumin as stabilizer.
  Label: FITC
  State: Liquid purified IgG fraction.
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A.
• Conjugation: FITC
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 1043 Human
• Background: The CD52 antigen is a remarkably small peptide that is heavily glycosylated, and attached to the cell surface membrane via a GPI link. The apparent molecular mass of the antigen on SDS-PAGE is 25-29kD. CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.
• Synonyms: HE5, CDw52