CD52 Rat Monoclonal Antibody [Clone ID: YTH34.5]

CAT#: SM2135P

CD52 rat monoclonal antibody, clone YTH34.5, Purified

Specifications

Product Data

• Clone Name: YTH34.5
• Applications: CT, ELISA, FC, IHC, IP, WB
• Recommended Dilution: Flow Cytometry: Use 10 µl of 1/20 diluted antibody to label 1 x 10e6 cells in 100ul.
  Immunohistochemistry on Frozen Sections: 1/20.
  Immunohistochemistry on Paraffin Sections: 1/20-1/40.
  Immunohistochemistry on Resin.
  Positive Control Tissue: Tonsil.
  ELISA.
  Western Blotting.
  Immunoprecipitation.
  Cytotoxic Assays: 50 µg/ml. Use Human serum as complement source.
• Reactivities: Human, Monkey
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human lymphocytes.
• Specificity: This antibody reacts with CD52 antigen.
  Humanised versions of CAMPATH-1 specific antibodies are currently in clinical trials for the treatment of a range of lymphoid malignancies.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction from Tissue Culture Supernatant
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 1043 Human
• Background: CD52 is a small peptide that is heavily glycosylated, and attached to the cell surface membrane via a GPI link. The apparent molecular mass of the antigen on SDS-PAGE is 25-29kD.
  CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.
• Synonyms: HE5, CDw52