PECAM1 Mouse Monoclonal Antibody [Clone ID: LCI-4]

CAT#: SM2146APC

PECAM1 mouse monoclonal antibody, clone LCI-4, APC

Product Images

Staining of porcine peripheral blood lymphocytes with Mouse Anti Pig CD31-APC.

Specifications

Product Data

• Clone Name: LCI-4
• Applications: FC
• Recommended Dilution: Flow Cytometry Use 10 ml of neat-1/10 diluted antibody to label 100 µl of whole blood or 10e6 cells in 100 µl.
• Reactivities: Human, Porcine
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Porcine CD31/human IgGFc fusion protein.
• Specificity: This anitibody recognises CD31, a 140 kD cell surface protein also called PECAM-1, expressed by various cell types, but particularly by endothelial cells.
  Clone LCI-4 has been shown to stimulate tyrosine phosphorylation of CD31 on endothelial cells.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.
  Label: APC
  State: Lyophilized purified IgG fraction.
  Label: Allophycocyanin
• Reconstitution Method: Restore with 1.0 ml distilled water.
• Concentration: 0.1 mg/ml
• Purification: Affinity Chromatography on Protein G.
• Conjugation: APC
• Gene Name: -
• Background: Cell adhesion molecules are a family of closely related cell surface glycoproteins involved in cell-cell interactions during growth and are thought to play an important role in embryogenesis and development. Neuronal cell adhesion molecule (NCAM) expression is observed in a variety of human tumors including neuroblastomas, rhabdomyosarcomas, Wilms’ tumors, Ewing’s sarcomas and some primitive myeloid malignancies. The intracellular adhesion molecule-1 (ICAM-1), also referred to as CD54, is an integral membrane protein of the immunoglobulin superfamily and recognizes the B2α1 and B2αM Integrins. PECAM-1 (platelet/endothelial cell adhesion molecule-1), also referred to as CD31, is a glycoprotein expressed on the cell surfaces of monocytes, neutrophils, platelets and a subpopulation of T cells. VCAM-1 (vascular cell adhesion molecule-1) was first identified as an adhesion molecule induced on human endothelial cells by inflammatory cytokines such as IL-1, tumor necrosis factor (TNF) and lipopolysaccharide (LPS). The KALIG gene encodes a nerve cell adhesion molecule (NCAM)-like protein and is deleted in 66% of patients with Kallmann’s syndrome, anosmia with secondary hypogonadism.
• Synonyms: PECAM-1, EndoCAM, GPIIA'