Chimaeric human IgM anti Nitrophenyl-hapten (NP) Human Monoclonal Antibody [Clone ID: THM]

CAT#: SM2185

Chimaeric human IgM anti Nitrophenyl-hapten (NP) human monoclonal antibody, clone THM, Supernatant

Specifications

Product Data

• Clone Name: THM
• Applications: ELISA
• Recommended Dilution: ELISA.
• Host: Human
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: Hapten, 4-hydroxy-3-nitrophenylacetyl (NP).
  Plasmids containing chimaeric heavy chain gene were fused with cells of the J558L mouse myeloma cell line.
• Specificity: The immunoglobulin heavy chain has been produced by the linking of the antigen-binding, variable region genes of a mouse hybridoma to human constant region genes by in vitro DNA recombination procedures. The resulting chimaeric antibody is subsequently expressed by the myeloma cell-line J558L after transfection. (The J558L cell-line self secretes a lambda light chain but no heavy chain). Thus a chimaeric human IgM antibody specific for NP has been produced.
• Formulation: 0,2 Tris/HCl, pH 7.4 containing 0.09% Sodium Azide as preservative and 5-10% foetal calf serum
  State: Supernatant
  State: Liquid Tissue Culture Supernatant.