CD11b Rat Monoclonal Antibody [Clone ID: 5C6]

CAT#: SM2199F

CD11b rat monoclonal antibody, clone 5C6, FITC

Specifications

Product Data

• Clone Name: 5C6
• Applications: FC
• Recommended Dilution: Flow Cytometry: The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
• Reactivities: Human, Mouse
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Thioglycollate-elicted Peritoneal Macrophages (TPM). Spleen cells from AO rats were fused with cells of the Y3 rat myeloma cell line.
• Specificity: This antibody reacts with complement type 3 receptor (CR3) and precipitates a heterodimer of 165 and 95kD. Clone 5C6 inhibits adhesion in vitro and inflammatory recruitment in vivo. Clone 5C6 also inhibits delayed hypersensitivity, inflammation and potentiates bacterial infections and inhibits type 1 diabetes. Partially prevents LPS induced myelomonocytic cell recruitment.
• Formulation: PBS, pH 7.2 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
  Label: FITC
  State: Liquid purified IgG
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.1 mg/ml
• Purification: Ion exchange chromatography
• Conjugation: FITC
• Background: CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes and is commonly used as a microglial marker in nervous tissue.
• Synonyms: ITGAM, CR3A, CR-3 alpha chain, Integrin alpha-M, MAC1