CD45 (PTPRC) Mouse Monoclonal Antibody [Clone ID: F10-89-4]

CAT#: SM21PT

CD45 (PTPRC) mouse monoclonal antibody, clone F10-89-4, Purified

Specifications

Product Data

• Clone Name: F10-89-4
• Applications: FC, IHC, IP
• Recommended Dilution: Immunohistochemistry on frozen sections: 1/500 - 1/1000. Immunohistochemistry on paraffin sections: 1/100 - 1/200; This product requires antigen retrieval using heat treatment prior to staining of paraffin sections; 1mM EDTA pH8.0 is recommended for this purpose.
  Immunoprecipitation.
  Flow Cytometry: 1/10 - 1/50; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human T lymphocytes
• Specificity: This antibody recognises the CD45 cell surface antigen, also known as the leucocyte common antigen (LCA).
  Clone F10-89-4 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes.
  This product is routinely tested in flow cytometry on human peripheral blood leucocytes
• Formulation: Phosphate buffered saline pH7.4 containing 0.09% Sodium Azide
  State: Purified
  State: Liquid purified IgG
• Concentration: lot specific
• Purification: Affinity chromatography on Protein G
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 5788 Human
• Background: CD45 is a complex molecule existing in a number of isoforms.
  Antibodies recognising a common epitope on all of these isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200