HLA Class II DR Rat Monoclonal Antibody [Clone ID: YD1/63.4.10]

CAT#: SM2201F

HLA Class II DR rat monoclonal antibody, clone YD1/63.4.10, FITC


USD 270.00

2 Weeks*

Size
    • 100 ug

Product Images

Specifications

Product Data
Clone Name YD1/63.4.10
Applications FC
Recommended Dilution Flow cytometry.
Immunohistochemistry on cryostat and Paraffin-embedded sections.
Reactivities Human
Host Rat
Isotype IgG2a
Clonality Monoclonal
Immunogen DAUDI cells
Donor: immunized DA rat spleen cells
Fusion Partner: Y3 Ag1.2.3 rat myeloma
Specificity Anti-human HLA-DR monoclonal antibody recognizes the HLA-DR (MHC class II) antigen.
Formulation PBS containing 0.02% sodium azide (NaN3) as preservativeand EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
Label: FITC
State: Liquid purified Ig fraction
Label: Fluorescein isothiocyanate isomer 1
Concentration 0,1 mg/ml
Purification Affinity chromatography on Protein G
Conjugation FITC
Synonyms HLA-DR, HLA class II histocompatibility antigen DR, MHC class II antigen DR
Note Protocol: FLOW CYTOMETRYANALYSIS:
Method:
1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-H cell separation medium.
2. Wash 2 times.
3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 μl of this suspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test).
4. To each tube, add 50μl of a 0.2-0.1μg dilution of this antibody per 10e6 cells.
5. Vortex the tubes to ensure thorough mixing of antibody and cells.
6. Incubate the tubes for 30 minutes at 4°C.
7. Wash 2 times at 4°C.
8. Resuspend the cell pellet in 50 μl ice cold media B.
9. Transfer to suitable tubes for flow cytometric analysis containing 15 μl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.
Media:
A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 μl of 2M sodium azide in 100 mls).
B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 μl of 2M sodium azide in 100 mls).
Results:
Tissue Distribution by Flow Cytometry Analysis:
Cell Concentration: 1x10e6 cells per test
Antibody Concentration Used: 0.1 μg/10e6 cells
Isotypic Control: FITC Rat IgG2a
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.