CD8A Mouse Monoclonal Antibody [Clone ID: CVS8]

CAT#: SM2207P

CD8A mouse monoclonal antibody, clone CVS8, Purified

Product Images

Staining of Equine peripheral blood lymphocytes with Mouse anti Horse CD8 antibody (Clone CVS8) followed by HRP conjugated Goat anti Mouse IgG HRP.

Specifications

Product Data

• Clone Name: CVS8
• Applications: FC, IHC, IP
• Recommended Dilution: Flow Cytometry: Use 10 µl of 1/25-1/200 diluted CD8 antibody to label 10⁶ cells in 100 µl.
  Immunoprecipitation.
  Immunohistochemistry on Frozen Sections.
• Reactivities: Donkey, Equine
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Equine PBMC.
  Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.
• Specificity: This monoclonal CVS8 antibody recognises the Equine homologue of the Human CD8 cell surface antigen which is expressed by a subset of T lymphocytes.
  A study undertaken using CVS8 to identify CD8 on several wild african equid species indicates that the CVS8 clone recognises Somali wild ass (Equus asinus) but not Grévy's Zebra (E. grevyi) or Hartmann's Mountain Zebra (E. zebra) (Ibrahim, S, 2007).
  Negatives Species: Zebra.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction from tissue Culture Supernatant
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
• Synonyms: CD8 alpha chain, CD8A, MAL